Figure 2. A summary of the screen for genes enhancing the pica phenotype of Q56-YFP.

(a) All available 5130 haploid non-essential knockouts in the MATa background were transformed with pQ56 plasmid and their colony growth pattern was evaluated individually. Sixty-eight strains were found to reduce the pica phenotype of pQ56. Six genes were targeted by two strains in the library, 30 diploid MATa/α strains were excluded, leaving 32 strains after the screen. By FACS analysis, the enhancers of pica were classified into three groups, based on their behaviour in response to pQ0 (black), pQ30 (blue) or pQ56 (red). For each group, the results for two representative strains are shown. (b) Representative images of deletion strains that are resistant to Q₅₆-YFP show that in many cases the pQ56-induced pica phenotype (lower row, left column) disappears and normal growth in comparison with pQ30 (upper row) is restored as shown for the strains hsp104Δ, scp160Δ, spt4Δ, pgd1Δ and vps69Δ. Scale bar represents 10 mm.