Table 1. Survivor colonies and growth on GdnHCl abolish the effect of polyQ proteins on septins.
Morphology | pQ0 normal colonies (% of cells) | pQ56 normal colonies (% of cells) | pQ56 large colony 1 (% of cells) | pQ56 large colony 2 (% of cells) | pQ56 large colony 3 (% of cells) | pQ56 GdnHCl-treated colonies (% of cells) |
---|---|---|---|---|---|---|
No structure | 22 | 17 | 20 | 25 | 24 | 45 |
Single ring | 66 | 36 | 68 | 68 | 62 | 43 |
Granular | 7 | 12 | 8 | 1 | 5 | 7 |
Single focus | 4 | 12 | 3 | 4 | 7 | 4 |
≥2 Foci | 1 | 23 | 1 | 2 | 2 | 1 |
The table summarizes the data (% of cells in each category) visualized in Fig. 5c and additional readouts for three weakly fluorescent survivor colonies, as well as GdnHCl-treated cells. The data indicate that pica escape and GdnHCl therapy equally abolish the pica phenotype also on a molecular level.