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. Author manuscript; available in PMC: 2013 Apr 3.
Published in final edited form as: Cell Rep. 2013 Feb 21;3(2):386–400. doi: 10.1016/j.celrep.2013.01.009

Figure 5. Systemic zinc deficiency increases the pro-inflammatory response to sepsis.

Figure 5

(A) Cytokine analysis of serum obtained from mice that were maintained on zinc deficient or corresponding control diet for three weeks, followed by cecal ligation and puncture (CLP). Samples were obtained 2 hrs after CLP. Zn, zinc; Zn-, zinc deficient; Ctrl/CLP: control (normal) diet plus CLP; Zn-/CLP, zinc-deficient diet plus CLP. (B) Labile zinc levels in peripheral blood monocytes at 2 hrs after CLP. Figure 6F describes the methodology. (C) Total zinc levels in the lung at 2 hrs after CLP, as measured by AAS. (D) Gene expression analysis of NF-κB target genes in mouse lung tissues. (E) Total proteins were extracted from lung and subjected to Western analysis. Densitometry is presented as a histogram to the right.