Figure 4.

Evaluation of the interaction of SRC1 cofactors with PPARγ in the presence of telmisartan or rosiglitazone. U2OS cells were cotransfected using the double-hybrid technique, and SRC1 interaction with PPARγ after treatment with telmisartan or rosiglitazone was evaluated. The data are expressed as the mean ± SEM, and represent a minimum of three independent experiments, with each data point run in triplicate for each experiment. Test was performed Analysis of Variance (ANOVA) and differences were considered statistically significant when the mean value with standard error was p ≤ 0.05. As in the transfection studies are performed co-transfection with β-galactosidase, to normalize the data and avoids the dispersion of the results. β-galactosidase has a different promoter reporter vector to thereby does not interfere with the results.