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. Author manuscript; available in PMC: 2013 Apr 3.
Published in final edited form as: Photochem Photobiol. 2010 Apr 7;86(4):752–756. doi: 10.1111/j.1751-1097.2010.00718.x

Figure 4.

Figure 4

Partial colocalization of NFK with mitochondria in HaCaT cells after photosensitization with rose bengal. HaCaT cells were incubated with 1, 2, or 5 µM rose bengal as indicated. After washing, cells were exposed to 20 min incubation under cool white fluorescent lights or were maintained in the dark as indicated. Cells were stained with anti-NFK antibody and anti-mitochondrial complex III antibody followed by Alexa 488 and Alexa 568 antibody. (a) DIC image (transmission microscopy), (b) anti-mitochondrial complex III (green), (c) anti-NFK (red), and (d) merge of (a), (b) and (c).