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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Microbes Infect. 2012 Feb 8;14(0):590–599. doi: 10.1016/j.micinf.2012.01.007

Fig. 1.

Fig. 1

PCR reactions to screen for mutant strains. Different sets of primers were designed to amplify the wild type and mutant alleles from each operon, as shown in panel A, and they were used to screen for mutants. Vertical arrows represent the beginning and the end of each mce operon and delimit the fragments that were deleted from the genome. Panel B shows PCR reactions done with one clone (called 25) of the sextuple mutant to confirm the deletion of each operon. Colony PCR was performed to this clone with the set of primers “A” to amplify the mutant allele and with the set of primers “B” and “C” to amplify the wild type allele from each mce operon. +A: positive control of the mutant allele using the delivery plasmid to mutate the corresponding operon as template. +B and +C: positive controls of the wild type allele using genomic DNA from M. smegmatis mc2 155 as template.