Figure 6.
Comparison of DR1 binding predictions with actual binding data. IC50 vales shown were determined for overlapping peptide series from Varicella (chickenpox) virus glycoprotein E antigen (A. Arvin, D. DeOliveira, L. Stern, unpublished), glucose decarboxylase autoantigen [65] and honeybee venom allergen [66]. Values were normalized with the influenza HA307–319 peptide common to all studies. Syfpeithi scores are on an arbitrary linear scale, IEDB scores reflect estimated IC50 values, and TEPITOPE scores are on a linear scale reflecting the binding relative to a test polyalanine-based peptide. None of the algorithms provide highly reliable predictions of binding affinity as measured by IC50 values, although consensus approaches based on these algorithms have proven useful in efficient prediction of T cell epitopes.