Figure 3. Bessel Plane SR-SIM Outperforms Widefield 3D SR-SIM in Densely Fluorescent or Thick Specimens.
(A) Three orthogonal maximum intensity projections of chromosomes in a live LLC-PK1 cell in anaphase, as seen by Bessel plane SR-SIM.
(B) Similar views in another live LLC-PK1 cell in anaphase, as seenby widefield 3D SR-SIM
(C) Lateral (left) and axial (right) maximum intensity projection views of a living early stage C. elegans embryo, as seen by Bessel plane SR-SIM.
(D) Similar views in another living C. elegans embryo, as seen by widefield 3D SR-SIM.
Scale bars: 2 µm in A, B; 5 µm in C, D.