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. Author manuscript; available in PMC: 2013 Jun 7.
Published in final edited form as: Cell. 2012 Dec 7;151(6):1370–1385. doi: 10.1016/j.cell.2012.10.008

Figure 5. In Vivo Karyotyping of a Mitotic U2OS Cell.

Figure 5

(A) Dual color imaging of chromosomes (green) and kinetochores (red) at six different time points from prometaphase to metaphase, taken from a series of 50 volumes acquired at 60 sec intervals. Three specific chromosomes (colored magenta, brown, and yellow) were identified by shape and tracked at all times.

(B) In vivo karyotyping by manual segmentation of all chromosomes at the initial time point. In each case, kinetochore pairs are shown correctly co-localized with their corresponding chromatids, and can be used to help identify specific chromosomes. The cell exhibits extreme polyploidy, with 76 diploids and one possible triploid (red arrow).

Scale bars: 5 µm in A; 2 µm in B. See also Movie S4.