Fig. 2.

Ad5 vector-induced immune responses. PBMC and sera were isolated from vaccinated and vector control RM at baseline (day 0) and during the course of 4 immunizations (A). Ad5 NAb endpoint titers peaked 2 weeks (day 14) after the first injection with either Ad5 [E1-, E2b-]-null (black circle) or multivalent Ad5 [E1-, E2b-]-SIV-gag/pol/nef/env (white square). Thereafter, Ad5 NAb levels decreased to newly established but higher baseline levels. Error bars indicate ± SEM. B. Ad5 CMI responses peaked on day 14 in vector control animals (black circle) and day 28 in Ad5 [E1-, E2b-]-SIV immunized RM. These responses were significantly higher in the vaccinated RM on the first day of Ad5 [E1-, E2b-]-gag/pol/nef/env immunization. *Days that there was a significant difference between the vaccinated and vector control RM (P<0.05).