Table 1.

Protein ratios from ¹⁸O-labeled peptides isolated from rat cardiac mitochondria

			APC vs. Control			Control vs. Ischemia			APC vs. APC+Ischemia
Gene Name	Gene Symbol	Uniprot ID	Unique Peptides	Ratio APC/Control	SE	Unique Peptides	Ratio Control/Ischemia	SE	Unique Peptides	Ratio APC/APC+Ischemia	SE
2,4-dienoyl-CoA reductase	Decr1	Q64591	4	2.66	0.289
Aconitate hydratase	Aco2	Q9ER34	10	1.12	0.054				3	1.44	0.156
ADP/ATP translocase	Slc25a4	Q05962	3	2.13	0.116	2	2.65	0.387
ATP synthase subunit alpha	Atp5a1	P15999	10	0.98	0.023	4	1.85	0.098	4	0.52	0.056
ATP synthase subunit b	Atp5f1	P19511				2	0.46	0.003
ATP synthase subunit beta	Atp5b	P10719	9	1.02	0.074	8	1.96	0.132	14	0.45	0.002
ATP synthase subunit d	Atp5h	P31399	4	0.69	0.369	2	1.24	0.002		0.51	0.032
ATP synthase subunit delta	Atp5d	P35434	2	0.89	0.007
ATP synthase subunit O	Atp5o	Q06647							2	0.85	0.372
Citrate synthase	Cs	Q8VHF5	2	1.79	0.076
Cytochrome b-c1 complex subunit 1	Uqcrc1	Q68FY0	2	0.58	0.168
Cytochrome b-c1 complex subunit 2	Uqcrc2	P32551	2	0.95	0.399	1	1.27	0.627	2	1.84	0.529
Cytochrome c	Cycs	P62898	3	0.89	0.006
Cytochrome c oxidase polypeptide 7A2	Cox7a2	P35171	2	1.24	0.568
Cytochrome c oxidase subunit 2	Mtco2	P00406	2	0.75	0.001	1	2.47	0.034	2	0.92	0.061
Cytochrome c oxidase subunit 5B	Cox5b	P12075	2	1.65	0.096
Electron transfer flavoprotein subunit alpha	Etfa	P13803	2	1.96	0.194			0.005
Electron transfer flavoprotein oxidoreductase	Etfdh	Q6UPE1				1	1.18
Glutamate dehydrogenase 1	Glud1	P10860	2	1.92	0.005
Isocitrate dehydrogenase [NADP]	Idh2	P54071	8	1.27	0.784	2	1.17	0.236
Malate dehydrogenase	Mdh2	P04636	11	1.18	0.002				2	0.63	0.007
NADH dehydrogenase 1 alpha subunit 4	Ndufa4	Q62425	2	0.34	0.007	1	0.52	0.045
NADH dehydrogenase 1 alpha subunit 9	Ndufa9	Q9DC69				1	0.45	0.002	3	0.78	0.318
NADH dehydrogenase 1 subunit C2	Ndufc2	Q9CQ54				2	0.67	0.209
NADH dehydrogenase flavoprotein 2	Ndufv2	P19234	3	1.97	0.178	2	0.57	0.194	2	0.66	0.093
NADH oxidoreductase 75 kDa subunit	Ndufs1	Q66HF1	4	1.45	0.085	2	1.35	0.005	2	1.46	0.063
Pyruvate dehydrogenase E1 subunit beta	Pdhb	P49432							3	0.59	0.003
Stress-70 protein	Hspa9	P48721	1	0.15	0.001
Succinyl-CoA ligase subunit alpha	Suclg1	P13086	2	0.34	0.002
Long-chain specific acyl-CoA dehydrogenase	Acadvl	P45953				2	1.03	0.003

Peptides were run on LC-MS/MS and identified using SEQUEST. Proteins enzymatically labeled with ¹⁸O and ¹⁶O were averaged over 3 replicates, and all identified peptides for each treatment pair. Treatments were: control rats (Control), rats preconditioned with isoflurane (APC), rats subjected to ischemia and reperfusion (Ischemia), and rats preconditioned with isoflurane before being subjected to ischemia and reperfusion (APC + Ischemia). Ratios in boldface indicate that the protein is significantly up- or downregulated based on the ratio being at least 2 SD away from a mean calculated from comparing 2 control rats against each other.