Fig. 3.

Proliferation responses. UCB CD34+ cells were cultured with IL3, IL6, SCF, TPO (36ST) (i.e., control). A: 36ST was added at day 0 along with one of the indicated cytokines. B: 36ST was added at day 0, and after 7 days of culture one of the indicated cytokines was added. The number of total nucleated cells was counted at day 14 of culture. The proliferation response is expressed as a percent of the control group. The line at the 100% level in each graph represents the proliferation response of control cultures. Data are shown as means ± SE in triplicate culture and are representative of 3 independent experiments from 3 biological samples. *P < 0.05.