Fig. 5.

Megakaryocyte colony-forming-unit (MK-CFU) formation. Cord blood (UCB) CD34+ cells were cultured for 12 days with IL3, IL6, SCF, TPO (36ST), plus one of the indicated cytokines in collagen containing media. CD34+ cells cultured with only 36ST were designated as controls. The number of colony-forming unit megakaryocytes (CFU-MK) was counted as described in materials and methods and normalized to the control group. The line at the 100% level in each graph represents the response of control cultures. Data are shown as means ± SE from duplicate cultures from 3 independent experiments. *P < 0.05.