Table 1.
CaV2.2/TN-XL parameters for HEK293 cells under TIRF imaging
| Fluorescence Parameters1 | Value |
|---|---|
| CaV2.2/TNXL Rmin | 1.10 (n = 20 cells, Fig. 2f) |
| CaV2.2/TNXL Rmid | 1.532 |
| CaV2.2/TNXL Rmax | 2.10 (n = 15 cells, Fig. 2f) |
| CaV2.2/TNXL α0 | 2.00 (n = 8 cells) |
| CaV2.2/TNXL α1 | 1.573 |
| Kinetic Parameters4 | Value |
|---|---|
| k01 | 174.4 ms−1 M−1 |
| k10 | 0.00239 ms−1 |
| k12 | 0.00174 ms−1 |
| k21 | 0.00309 ms−1 |
| k23 | 0.126 × 106 ms−1 M−2 |
| k32 | 1/733 ms−1 |
1
Fluorescence parameters obtained following experimental procedures used to characterize TN-L1548, unless specified otherwise.
2,4
Set by global fitting of Fig. 4e scheme to data Figs. 4a, 4c, and 4d.
3
Linear interpolation on R values. Explicitly, α1 = α0 − (Rmid − Rmin)/(Rmax − Rmin).