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. Author manuscript; available in PMC: 2013 Sep 11.
Published in final edited form as: Curr Biol. 2012 Jul 5;22(17):1545–1553. doi: 10.1016/j.cub.2012.06.029

Figure 2. The PH-GRAM Domain and Phosphatase Activity Are Required for Ymr1 Localization to the PAS.

Figure 2

(A) WT cells expressing RFP-Ape1 and Ymr1-GFP (ECY202) were incubated with rapamycin for 3 hr before collecting images.

(B) Cells expressing RFP-Ape1 and Sjl3-GFP (AVY070) were treated and imaged as in (A).

(C) The ymr1Δ RFP-Ape1 (ECY207) strain carrying integrated pYMR1GFP(406) (Ymr1-GFP), pNΔYMR1GFP(406) (NΔYmr1-GFP), or pYMR1C397SGFP(406) (Ymr1C397S-GFP) were starved for 3 hr in SD-N medium before imaging. Arrows highlight colocalization. DIC, differential interference contrast.

(D) Quantification of the degree of RFP-Ape1 colocalization with the GFP-tagged proteins in the samples imaged in (C). The graphs represent the average of two experiments ± SEM.

Scale bars represent 5 μm. See also Figure S2.