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. 2013 Feb 22;14(4):356–363. doi: 10.1038/embor.2013.16

Figure 1.

Figure 1

Upregulation of TERF2 mRNA when activation of the canonical Wnt/β-catenin signalling pathway is more pronounced. (A) TERF2 mRNA expression in 56 hepatocellular carcinomas with known β-catenin mutations. P-value determined according to a Mann–Whitney U-test. Error bar represents mean±s.e.m. (B) Relative TERF2 mRNA levels in normal intestine and adenocarcinoma samples from APC+/+ and APC1638N/+ mice (n=6 for each type of mouse). The values are normalized to tissues of APC+/+ mice. The P-value was determined using Bonferroni multiple comparison test. Error bar represents mean±s.e.m. for six independent experiments. (C) Reverse transcription–quantitative PCR analysis of the expression of Ctnnb1, c-Myc and TERF2 genes in skin samples from the indicated newborn mice treated topically with Tamoxifen between days 0.5 and 1.5 and euthanized at day 3. The P-value was determined using Wilcoxon matched-pairs signed rank test. Error bar represents mean±s.e.m. (D) Representative images of immunofluorescence analysis using antibodies directed against TRF2 or β-catenin of skin samples of the indicated mice treated with Tamoxifen. The white circles show examples of cells that still express a high level of β-catenin and of TRF2. The bar represents 15 μm. DAPI, 4′-6-diamidino-2-phenylindole.