Figure 3.
The TCF–LEF sites of the human TERF2 gene are capable of transactivating a luciferase reporter gene in a β-catenin-dependent manner. (A) The various reporter constructs derived from the pLG-3/Prom plasmid (for the TERF2 Reg sequence) and pLG-3/basic (for the TERF2 Prom sequence). TCF–LEFmut: all the TCF–LEF sites are inactivated by mutation. TOP-Flash and FOP-Flash are positive and negative controls, respectively, for β-catenin/TCF transactivation. Data show average relative luciferase activity in HCT116 cells and represent the mean±s.d. (n=6). Co-transfected plasmids overexpressing activated β-catenin and Tcf1E are identified by black/white scales. (B) Efficiency of inhibition of Ctnnb1 mRNA expression on transfection of two different short interfering RNAs (siRNAs) directed against Ctnn1b mRNA (#1 and #2). SiCtl is a scramble control siRNA. (C) HCT116 cells transfected with various siRNAs as in A. Statistical significance of data was assessed using Bonferroni multiple comparison test. For all panels, error bar represents the mean±s.e.m. for six independent experiments.