FIG. 4.

Detection of apoptosis in SDT-treated U937 cells using flow cytometry. The cells were double stained with Annexin V-PE and 7-AAD as described in the Methods section after 4 hours of incubation after SDT treatment. (A) Control group with untreated cells; (B) cells were treated with 10 μg/mL HMME alone; (C) cells were irradiated with 1 W/cm² ultrasound alone; (D) cells were irradiated with 1 W/cm² ultrasound in the presence of 10 μg/mL HMME. SDT, mediated sonodynamic therapy.