FIG. 7.

Measurement of intracellular ROS in U937 cells. The cells were labeled with DCFH-DA, and the fluorescence intensity of DCF in the cells was detected by flow cytometry. Control, control group with untreated cells; HMME, cells with 10 μg/mL HMME alone; ultrasound, cells were irradiated with 1W/cm² ultrasound alone; ultrasound+HMME, cells were irradiated with 1 W/cm² ultrasound in the presence of 10 μg/mL HMME. ROS, reactive oxygen species; DCFH-DA, 2′,7′-dichlorodihydrofluorescein-diacetate.