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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2012 Jul;25(4):493–505. doi: 10.1111/j.1755-148X.2012.01005.x

Figure 4. Synergistic effect of blockade CCL2 and MMPs on MCMI-Mϕ induced melanoma invasion.

Figure 4

1205Lu melanoma cells were seeded into matrigel precoated Transwells and were incubated for 18 hr. Conditioned medium from 1205Lu-Mϕ or control medium were added to the bottom chamber. Migrated cells were stained (using a Diff-Quick staining kit) and photographed. (A) Control medium. (B) 1205Lu-Mϕ supernatant. Blockade of MMP or CCL-2 alone marginally increased melanoma cell invasion (C&D). Blockade of both MMPs and CCL-2 resulted in the significant inhibition of melanoma invasion (E). Data are representative of 3 independent experiments. (F) Summary of all data. *** P<0.01.