Figure 5.

Increased kinetics of DSBs repair after radiation in pmiR-210 A549 cells. (a) Induction of 53BP1 foci in pmiR-Ctl and pmiR-210 A549 cells following 0–8 Gy at 15 min; scale bar, 7.3 μm. (b) Histograms show the quantification of the average foci per nucleus from at least three independent measurements. (c) 53BP1 colocalizes with the canonical DSB marker γH2AX in pmiR-Ctl and pmiR-210 A549 cells at 48 h following 8 Gy; scale bar, 7.3 μm. (d) Histograms show the quantification of the foci per nucleus on average from at least three independent measurements in a time-responsive manner (18, 48 and 72 h after irradiations). (e) A549 cells, pmiR-Ctl and pmiR-210 were cultured for 2 days, and at this point, treated with cisplatin (1 μg/ml) for 24 h or docetaxel (25 nM) for 4 h, or bleomycin for 2 h (10 μg/ml). Then, cells were trypsinized and plated in triplicate (1000 cells per well in 6-well plates) for clonogenic survival. Cells were allowed to form colonies during 10 days. At this time, they were fixed and stained with crystal violet and counted using ImageJ software. Data represent the average of two independent experiments. *P<0.05 and **P<0.01