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. 2013 Mar 21;4(3):e553. doi: 10.1038/cddis.2013.69

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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HSF1 expression is decreased in TO cells with impaired cellular availability of B12. In TO cells, the HSF1 protein level is significantly reduced compared with that in control cells (P<0.01). In addition, the HSF1 protein present in TO cells is overwhelmingly acetylated (P<0.01) (a). Immunofluorescence staining of the HSF1 protein (green) in TO and OT cells reveals more TO than OT cells with weak nuclear HSF1 staining (P<0.0001) (b). Data were compared by one-way ANOVA with Fisher's test, with n=8 in each group, and presented as means±S.E. (*P<0.05; **P<0.01)