Figure 7.

The effect of the activator and inhibitor of SIRT1 on the expression of ER stress markers is modulated by vitamin B12 in TO and OT cells. SIRT1 inhibitor, EX527, reduces the SIRT1 protein expression in both cell types (P<0.01) and B12 supplement reverses its effects on ER stress transducers, including P-PERK (P<0.05) and P-IRE1α (P<0.05 for all comparisons). B12 reverses also the expression of BiP after EX527 treatment in TO and OT cells (P<0.05 for both cell types). Data were compared by one-way ANOVA with Fisher's test, with n=8 in each group, and presented as means±S.E. (*P<0.05 and **P<0.01)