Figure 2.

Caspase-3 is activated independent of apoptosis induction during erythroid differentiation of K562 cells. (a) The expression of Caspase-3, Caspase-7, PARP, XIAP and GAPDH in MCF-7 cells (lane 1) and K562 cells induced for erythroid differentiation for up to 8 days with sodium butyrate (lanes 2–6) or treated with puromycin for 16 h (lane 7) was analyzed in western blots with specific antibodies. (b) Caspase-Glo 3/7 assay of K562 cells induced for erythroid differentiation for up to 8 days or treated with puromycin for 16 h. The Caspase-3 inhibitor Ac-DMQD-CHO was added at the final concentration indicated. (c) Flow cytometric analysis of an AnnexinV-FITC and propidium iodide staining of K562 cells induced for erythroid differentiation for up to 8 days or treated with puromycin for 16 h