Figure 4.

Ectopically expressed Caspase-3 restores hnRNP K cleavage in MCF-7 cells. (a) K562 cells, MCF-7 cells transfected with pcDNA3.1 (MCF-7(pcDNA3.1)) or with pcDNA3.1-Caspase-3 (MCF-7(Caspase-3)) were treated with puromycin for 16 and 24 h. Western blot analysis with antibodies directed against Caspase-3, Caspase-7, PARP, hnRNP K, GAPDH. The dotted line indicates extended exposure of the hnRNP K cleavage product. (b) Caspase-Glo 3/7 assay of lysates from K562, MCF-7(pcDNA3.1) and MCF-7(Caspase-3) cells treated as in (a). Addition of Ac-DMQD-CHO at a final concentration of 10 μℳ as indicated