Figure 2.

Effect of TiO₂ on respiration. (a) OCR of HaCat cells exposed sequentially to each modulator of mitochondrial activity (oligomycin, FCCP and Rotenone) in presence of different concentrations of TiO₂ for 24 h. A representative experiment out of five performed, is shown. (b) Basal cellular respiration for each concentration used, as from experiment in A. (c) Effect of TiO₂ on rotenone-sensitive OCR, as from experiment in (a). Data in (b and c) represent mean±S.E.M. of three independent experiments. Statistical P-values in (b) and (c) are shown, compared with control. See also Supplementary Figures 1 for relative cumulative effect on three independent experiments and for ECAR