Figure 4.

Vector constructs and expression efficiency after electroporation. After CBX1-LEDGF_325-530(D366N) mRNA electroporation, X-CGD PLB-985 cells were transduced with LV_gp91 or LV_eGFP and subsequently differentiated into granulocytes. (a) Schematic representation of transfer plasmids used for vector production. The SFFV promoter drives gp91 or eGFP expression. All lentiviral vectors are based on HIV-1_NL4.3. (b) Flow cytometry was used to determine expression levels of gp91 (7D5 antibody) in electroporated cells. CBX1, heterochromatin protein 1β eGFP, enhanced green fluorescent protein; gp91, glycoprotein 91; LEDGF/p75, lens epithelium-derived growth factor; LTR, long terminal repeat; LV, lentiviral vector; SFFV, spleen focus-forming virus; SIN, self-inactivating; X-CGD, X-linked chronic granulomatous disease; Ψ, packaging signal.