Figure 2. Spiny lobster IR25a and IR93a transcripts can be localized to the ORNs.

In situ hybridization of vibratome sections of gelatin embedded lobster olfactory tissue. Antisense probes for PargIR25a and PargIR93a label most, if not all, mature ORNs. In contrast, no labeling was detectable with probes for other IRs. Specifically labeled cell bodies are indicated with an arrow in the first panel. Representative sections labeled with antisense probes for PargIR8a and PargIR9 are shown. No specific labeling was detected with the sense probes for the same gene regions. The cuticle in each section is non-specifically labeled.