Table 1. Comparison of predicted amino acid sequences of lobster and Drosophila IRs.
PargIR25a | PargIR8a | PargIR93a | |
DmelIR25a | 51.5% | 24% | 15.4% |
DmelIR8a | 24.2% | 39.4% | 14.1% |
DmelIR93a | 13% | 11.8% | 23.9% |
PargIR25a | 100% | 24.7% | 15.7% |
PargIR8a | 24.7% | 100% | 14.4% |
PargIR93a | 15.7% | 14.4% | 100% |
HameIR25a | 82% | 25.2% | 15% |
HameIR8a | 25.9% | 79.3% | 15% |
Drosophila (Dmel), Panulirus argus (Parg) and Homarus americanus (Hame) IR predicted amino acid sequences were compared. Geneious software, version 5.6.6 created by Biomatters (available from http://www.geneious.com/) was used to translate putative protein sequences and align sequences (using the Geneious ClustalW plug-in) to generate percent amino acid identity. Accession number include KC595306 for PargIR25a, KC595307 for PargIR8a, KC595308 for PargIR93a, AY098942.1 for HameIR25a, NM_135019.2 for DmelIR25a, NM_167185.1 for DmelIR8a, and NM_142666.4 for DmelIR93a. The HameIR8a sequence is unpublished (generously shared by Dr. Timothy McClintock).