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. 2013 Apr 3;8(4):e60545. doi: 10.1371/journal.pone.0060545

Figure 7. MicroRNA-301a regulated expression of Kv4.2.

Figure 7

The expression of miR-301 in db/db and Wt mouse right and left ventricles were assessed by Taq-man PCR (A). Rat cardiomyocytes (H9C2) were transfected with either scrambled or miR-301 inhibitor (50 nM) for different time points 24, 48 and 72 hours and miR-301a expressions were quantified using TaqMan assay (B). The bars labeled with 72‡ are the cells transfected with inhibitor (50 nM) for 72 h with additional supply of inhibitor at 48 h. The expression of Kv4.2, Kv4.3 KChIP2, Irx5, and NFkB were quantified using qRT-PCR for the cells transfected with miR-301a inhibitor or scrambled inhibitor for 24 h (C), 48 h (D) and 72 h (E). All values are normalized with housekeeping gene (U6 RNA for TaqMan and HPRT for qRT-PCR) and plotted as mean (±SEM) of n = 3–6. * represents p≤0.05, ** with p≤0.005, and *** with p≤0.0005.