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. 2013 Apr 3;8(4):e61027. doi: 10.1371/journal.pone.0061027

Figure 1. Effect of NEDD4-like family E3 ubiquitin ligases on LATS1 stability.

Figure 1

A) Dose-dependent degradation of LATS1 by E3 ubiquitin ligases. LATS1-FLAG plasmids were transfected alone (0.2 µg) or together with increasing amounts [0.05, 0.1, 0.2, 0.4 µg] of WWP1 plasmids into COS7 cells. The levels of LATS1-FLAG and WWP1-myc were detected by western blotting using anti-FLAG and anti-Myc antibodies, respectively. B) Knockdown of NEDD4-like family E3 ubiquitin ligases on LATS1 stability. About 100 nM of siRNA targeting scrambled RNA (negative control) or each E3 ligase was transfected into MCF7 or T47D cells in a 6-well plate. Two days after transfection, proteins were extracted and subjected to western blot analysis of each E3 ligase, LATS1, and β-actin (internal loading control).