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. 2013 Apr 3;8(4):e55943. doi: 10.1371/journal.pone.0055943

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Plasmids containing pol (pNL4-3) or the 2-LTR junction sequence were spiked into varying amounts of cellular background (salmon sperm) DNA prior to droplet formation. The figure shows the measured copy number as a percentage of the value expected from A₂₆₀/A₂₈₀ absorptivity of the plasmid. Loading of over 1.5 µg of DNA per well greatly reduced the measured copy number for all samples tested. Above 3 µg/well, droplet formation was also inhibited (not shown).