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. 2013 Apr 3;8(4):e59335. doi: 10.1371/journal.pone.0059335

Figure 4. Changes in mTOR related p-4EBP1 expression in samples of ALL patients after in vivo chemotherapy and in cultured ALL cells treated with rapamycin in vitro.

Figure 4

(a) P-4EBP1 OD is reduced by chemotherapy, but re-increases during relapse in ALL patient samples. Data is shown from 2 representative patients with good prognosis and 2 patients with poor prognosis at day 0, day 33, after three months of treatment, and at relapse (ELISA). (b) In vitro rapamycin sensitivity is variable in different human ALL samples (n = 4). P-4EBP1 levels variably decrease or increase after in vitro rapamycin treatment (50 ng/ml, 24 h) in isolated ALL cells (ELISA). A decrease in p-4EBP1 was associated with concomitant induction of apoptosis by rapamycin (>50% apoptotic cells in the first two samples; data not shown). (The first three samples were cultured from cells isolated from primary ALL cases and one at relapse.) *- P<0.05.