Figure 2. Heparanase is essential for inducible gene transcription in T cells. Jurkat T cells were transfected with validated heparanase siRNAs (RNAi1, RNAi2, RNAi3) or a negative control siRNA (Mock). At 48 h post-transfection, Jurkat T cells were either left untreated (NS) or stimulated with PI for 2 h (ST). Total RNA was extracted and TaqMan real-time PCR performed for CD69 (A), IL-2 (B), IFN-γ (C), TNF-α (D), MMP-2 (E) and MMP-14 (F). Results are plotted as arbitrary copies and normalized to GAPDH (A-D) or cyclophilin A (E-F). (G) Jurkat T cells were transfected with empty control vector (Ctrl) or a heparanase wild-type overexpression construct (HPSE) and at 48 h post-transfection cells were processed as above. Data represent the mean ± SE of three independent experiments and (*) indicates a non-significant change.