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(A) ChIP assays of Utx and the indicated histone modifications on the Hoxb1 promoter at the indicated times during differentiation. (B–F) Utx binding to promoter regions of ectoderm (Msi1, Sox1); mesoderm (Flk1, T) and endoderm (FoxA2) developmental regulators as assayed by ChIP. “% input” represents (bound/input material x 100). Error bars represent SD, n = 3 independent assays. (*p<0.05; ***p<0.0005, two tailed Student’s test).
