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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Mutat Res. 2012 Dec 17;0:26–32. doi: 10.1016/j.mrfmmm.2012.12.001

Figure 5.

Figure 5

Western blot analysis of p-Chk1(Ser345) and total Chk1 protein in whole cell lysates of WT (top panels), LN428/UNG-KD (middle panels), and LN428/SMUG1-KD (bottom panels) cells following treatment with 50 μM 5-FU for 24 h and recovery in drug free medium for the times indicated. β-Actin was used for the loading control. The experiment shown is representative of 3 independent experiments.