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. 2013 Apr 3;8(4):e60218. doi: 10.1371/journal.pone.0060218

Figure 2. A. Low power retinal section from eye 8-OD through the optic nerve (ON).

Figure 2

Green shows GFP expression and blue is DAPI staining. Note that there is GFP expression in axons within the optic nerve and also within the part of the inner retina corresponding to the nerve fiber layer. The section is taken through the dorsal vasculature radiating from the optic nerve head and the blood vessels are the reason that the nerve fiber layer is not a continuous layer in this section. This region is away from the subretinal injection bleb which is why only the nerve fiber layer is expressing GFP and the outer retina is not. Inl = inner nuclear layer; onl = outer nuclear layer. Size bar 100 µm. B. Section through the optic nerve from eye 2-OD. This eye received an injection of AAV2(quadY-F). Green is GFP expression, blue DAPI staining. GFP expression is seen in the axons of the optic nerve. Size bar 250 µm.