Skip to main content
. 2013 Apr 3;8(4):e60027. doi: 10.1371/journal.pone.0060027

Figure 2. Downregulation of CACNA2D3 is associated with promoter methylation and allele loss.

Figure 2

(A) Representatives of CACNA2D3 expression (bottom) and promoter methylation status (upper) in primary ESCC cases. (B) Restoration of CACNA2D3 mRNA expression could be observed in HKESC1 and KYSE180 after demethylating agent 5-AZA treatment. The promoter methylation status was also compared. (C) Representative of allele loss at SNP site rs589281 in three ESCC tumors. The SNP site was indicated by red arrows. (D) Pattern of CACNA2D3 expression, promoter methylation and allele loss in 27 informative ESCC cases. (E) 2 centrimere signals (green, indicated by narrow arrows) and 1 CACNA2D3 signal (red, indicated by wide arrow) were detected in KYSE410 cells; Tumor cells with CACNA2D3 deletion are indicated by arrows (100×objective).