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. Author manuscript; available in PMC: 2013 Apr 4.

Published in final edited form as: J Ethnopharmacol. 2012 Jan 2;140(1):107–116. doi: 10.1016/j.jep.2011.12.043

Fig. 5 — (A) An NF-κB reporter cell line derived from RAW 264.7 cells was treated with the indicated amounts of DS for 16 h prior to LPS treatment for 16 h. Luciferase activity was normalized by the amount of total proteins in cell lysate (*** P< 0.005, compared to the LPS-treated (the 3^rd column)). (B) HEK 293 cells were transfected with either 0.1 μg of an empty plasmid (pcDNA 3.1) or a plasmid encoding IKK-β, along with 0.05 μg of NF-κB reporter construct. Total amount of transfected DNA was 0.2 μg. At 24 h after transfection, the transfected cells were treated with the indicated amounts of DS for 16 h. Luciferase activity was normalized to tk-Renilla luciferase activity. No significant effect was detected. Data represent the mean±SEM of three independent experiments.

Fig. 5 — (A) An NF-κB reporter cell line derived from RAW 264.7 cells was treated with the indicated amounts of DS for 16 h prior to LPS treatment for 16 h. Luciferase activity was normalized by the amount of total proteins in cell lysate (*** P< 0.005, compared to the LPS-treated (the 3^rd column)). (B) HEK 293 cells were transfected with either 0.1 μg of an empty plasmid (pcDNA 3.1) or a plasmid encoding IKK-β, along with 0.05 μg of NF-κB reporter construct. Total amount of transfected DNA was 0.2 μg. At 24 h after transfection, the transfected cells were treated with the indicated amounts of DS for 16 h. Luciferase activity was normalized to tk-Renilla luciferase activity. No significant effect was detected. Data represent the mean±SEM of three independent experiments.