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. Author manuscript; available in PMC: 2013 Apr 4.
Published in final edited form as: Oncogene. 2011 Dec 5;31(31):3569–3583. doi: 10.1038/onc.2011.547

Figure 2.

Figure 2

Morphological and transcriptional effects of HER2 overexpression and EGF treatment of mammary spheroids. (a) Acini of HER2-overexpressing MCF10A cells were grown for 8 days in Matrigel in the absence or presence of EGF, before immunostaining with 4,6-diamidino-2-phenylindole (DAPI) and with the indicated antibodies (scale bar, 50 μm). (b) Acini of HER2-overexpressing MCF10A cells were grown in the absence or presence of EGF for the indicated time intervals, before immunoblotting (IB) with the indicated antibodies. (c) RNA expression levels of genes whose variation of expression during acinar morphogenesis is different in untreated versus EGF-treated MCF10A/HER2 cells and in MCF10A (control) cells. Expression levels were normalized and the genes grouped according to their known functions: adhesion, BMP/TGFβ signaling and angiogenesis/response to hypoxia.