Fig. 2.

Immunohistochemical sections of proliferating nuclear cell antigen (PCNA) expression in the tumor tissue (a and b). The PCNA-positive cells are stained brown (a HAI sham group, b HAI CE + BE + OX). Panels c and d show the data of the immunohistochemical analysis of PCNA (given as index: 0≤ 1 %, 1 = 1–10 %, 2 = 10–30 %, 3 = 30–50 %, 4 ≥ 50 % of PCNA-positive cells) in the tumor of animals undergoing HAI (c) or sCHT (d) of cetuximab plus bevacizumab (CE + BE), oxaliplatin (OX) or the combination of all three drugs (CE + BE + OX). Animals undergoing HAI or systemic application with saline served as controls (sham). Data are given as mean ± SEM; *p < 0.05 versus sham. ×200 magnification