Fig. 4.

Immunohistochemical sections of PECAM-1-positive blood vessels of the tumor tissue (a and b). PECAM-1-positive blood vessels are stained brown (a HAI sham, b HAI CE + BE + OX). Panels c and d show the quantitative analysis of PECAM-1-positive blood vessels in the tumor (given as number per HPF) of animals undergoing HAI (c) or sCHT (d) of cetuximab plus bevacizumab (CE + BE), oxaliplatin (OX) or the combination of all three drugs (CE + BE + OX). Animals undergoing HAI or systemic application of saline served as controls (sham). Data are given as mean ± SEM; *p < 0.05 versus sham; ^# p < 0.05 versus corresponding sCHT. ×400 magnification