Figure 3.

Representative example of outcome of the different steps in dendrite image processing. (A) Raw data—image stack corresponding to morphological structure (in this case resulting from eGFP expression). (B) The same image stack following filtering; (C) filtering and segmentation; (D) filtering and segmentation, followed by a second round of filtering; (E) two sequential rounds of filtering and segmentation. Images shown in (A–E) are z-projections calculated from all focal planes. (F) Image after skeletonization of resulting segments [from data shown in (E)]. Scale bar, 10 μm.