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. 2013 Mar 1;32(7):982–995. doi: 10.1038/emboj.2013.37

Figure 1.

Figure 1

Expression of the homeodomain-containing protein HLX1 controls senescence. (A) Expression of HLX1 extends cellular lifespan. IMR-90 cells (passage 17) were infected with pBABE or pBABE-HLX1 vectors and after selection, cells were seeded for colony formation assays (right). Immunoblot (IB) showing HLX1 expression is shown as a control (left). (B) Growth curves of IMR-90 cells infected with pBABE or pBABE-HLX1 vectors. (C) Effect of HLX1 expression on SA-β-Gal activity and SAHF formation in IMR-90 cells. Cells were analysed at passage 27 (marked by an arrow in B). shp16, shRNA targeting p16INK4a. (D) Effect of point mutations in the HLX1 homeodomain on cell growth and senescence in IMR-90 cells was assessed by crystal violet (top) and SA-β-Gal staining (bottom). Cells are shown at passage 24, as explained in Supplementary Figure S2. (E) Expression of HLX1 diminishes the arrest observed during OIS. Crystal violet staining of IMR-90 ER:RAS cells infected with the indicated vectors and treated with 4OHT where indicated (+4OHT).