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. 2013 Mar 1;32(7):982–995. doi: 10.1038/emboj.2013.37

Figure 6.

Figure 6

HLX1 associates with HDAC1 and the PRC2. (A) In situ PLA of endogenous proteins in IMR-90 shows an association between HLX1 and the PRC2 and HLX1 and HDAC1. A rabbit antibody against HLX1 and the indicated mouse antibodies were used to detect the protein complexes. Representative images (bottom) and a quantification of the percentage of cells displaying an interaction (top) are shown. Cells presenting 10 or more nuclear spots were scored as positive. Blue: DAPI; Red: PLA signal. (B, C) HLX1 co-immunoprecipitates with the PRC2 and HDAC1. HEK 293T cells were transfected with plasmids expressing HLX1 and a FLAG-tagged version of SUZ12 or EED (B) or HDAC1 (C) as indicated. Total lysates or FLAG immunoprecipitates were prepared and immunoblots conducted using antibodies recognizing HLX1 or the FLAG epitope. Specific bands are noted by an arrow, while non-specific bands are denoted by *. WB, western blot; IP, immunoprecipitation. (D) Co-immunoprecipitation between endogenous HLX1 and SUZ12. HeLa nuclear extracts were used to conduct IPs using antibodies against HLX1 or IgG controls. Proteins were resolved by SDS–PAGE and HLX1 or SUZ12 detected by immunoblotting.