Figure 7.

Tethered subunits of the CCR4–NOT complex promote PABP dissociation in the absence of deadenylation. (A–E) A tethering assay was performed using the F-Luc-5BoxB-(A)₉₅-HhR reporter and the indicated λN-HA-tagged proteins. (A) Firefly luciferase activity was normalized to that of the Renilla luciferase and set at 100 in cells expressing the λN-HA peptide (white bars). (B) Northern blot of representative RNA samples. The first lane of the upper panel shows the migration of a corresponding reporter lacking the internal poly(A). (C–E) The association of the F-Luc-5BoxB-(A)₉₅-HhR reporter with endogenous eIF4E, eIF4G and PABP in the absence (white bars) or presence of the indicated proteins (coloured bars) was analysed as described in Figure 1.

Source data for this figure is available on the online supplementary information page.

Source Data for Figure 7 ^{(784.4KB, pdf)}