Figure 5.

Reactivation of radial NSC proliferation and hippocampal neurogenesis by conditional deletion of p57 in aged mice. (A) Tamoxifen was administered to young adult (2-month-old) and aged (14- to 15-month-old) mice. Animals were sacrificed 17 days after the first tamoxifen administration. (B–D) Quantification of the number of PCNA⁺ radial NSCs (B), the proportion of PCNA⁺ radial NSCs (C), and the number of radial NSCs (D) in the DG of young adult (left) and aged mice (right). n=5 and 3 for young adult control and p57 cKO, respectively, and n=4 and 4 for aged control and p57 cKO, respectively. (E–G) Quantification of PCNA⁺ cells (E), Tbr2⁺ IPCs (F), and DCX⁺ neuroblasts/immature neurons (G) in the SGZ/GCL of aged mice. (H) Representative images of DCX⁺ cells in aged control and p57 cKO mice. Scale bar, 100 μm. Values represent mean±s.e.m. *P<0.05; Mann–Whitney U-test in (C) and (E). Others, ***P<0.001; **P<0.01; *P<0.05; Student’s t-test.