Figure 6.

Excessive loss of radial NSCs by long-term p57 deletion. (A) Experimental design for the quantification of the number and the proliferation of radial NSCs and the generation of new neurons after long-term deletion of p57. Tamoxifen was administered repeatedly from 1 month to 17 months after birth, and then mice were sacrificed at 24 months after birth. (B–H) Quantification of the number of radial NSCs (B), the number of PCNA⁺ radial NSCs (C), the proportion of PCNA⁺ among radial NSCs (D), the number of PCNA⁺ cells (E), the number of Tbr2⁺ IPCs (F), and the number of DCX⁺ neuroblasts/immature neurons (G), and GFAP and S100β double-positive cells (H) in the SGZ/GCL of control (n=4) and p57 cKO (n=4). *P<0.05; Student’s t-test in (B). *P<0.05; Mann–Whitney test in (E–G). In (C, D), P=0.093; Mann–Whitney test. (I) Experimental design for the quantification of newly generated mature neurons and astrocytes after long-term p57 deletion. Tamoxifen was administered repeatedly from 1 month to 17 months after birth, and then animals were sacrificed at 27–30 months after birth (36 days after 10 days of BrdU injections). (J, K) Quantification of the number of BrdU⁺ cells expressing NeuN (J) and S100β (K) in the SGZ/GCL of control (n=7) and p57 cKO mice (n=4). *P<0.05; Student’s t-test. Values represent mean±s.e.m.