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. Author manuscript; available in PMC: 2013 Apr 4.
Published in final edited form as: Cell Metab. 2012 Jun 6;15(6):838–847. doi: 10.1016/j.cmet.2012.04.022

Figure 5. Chronic NR supplementation increases plasma and intracellular NAD+ content in a tissue-specific manner.

Figure 5

Tissues from C57Bl/6J mice were collected after 16 weeks of HFD supplemented with either water (as vehicle; white bars) or NR (400 mg/kg/day; black bars). (A) NAD+ levels were measured in acidic extracts obtained from different tissues. (B) NADH and NAM levels were measured in gastrocnemius muscle. (C) Quadriceps muscle protein homogenates were obtained to test global PARylation, PARP-1 and Nampt protein levels. (D) Total mRNA was isolated from quadriceps muscles and the mRNA levels of the markers indicated were measured by RT-qPCR. Throughout the figure, all values are expressed as mean +/− SD. * indicates statistical significant difference vs. respective vehicle treated group.