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. Author manuscript; available in PMC: 2013 Apr 4.
Published in final edited form as: Cancer Res. 2008 May 1;68(9):3396–3404. doi: 10.1158/0008-5472.CAN-07-6396

Figure 5.

Figure 5

Combined temozolomide and IR results in the greatest inhibition of tumor growth and fluorescence emitted from human malignant glioma cells grown in zebrafish embryos. Embryos containing U251:RFP tumors were exposed to temozolomide alone (TMZ Only), irradiated with 10 Gy, or temozolomide followed by 10 Gy IR (10 Gy and 10 Gy + TMZ, respectively) and imaged on sequential days. A to C, images taken under fluorescence of representative zebrafish embryos at 1 dpf (A), 3 dpf (B), or 5 dpf (C). D, the fluorescence emitted by the tumor masses of each treatment group was measured at all three time points. Columns, mean levels of emitted fluorescence in arbitrary fluorescence units; bars, SE. These data were derived from three replicate experiments encompassing the following numbers of embryos: n = 16 for the “TMZ only” group, n = 18 for the “10 Gy” group, and n = 19 for the “10 Gy + TMZ” group.